| Equilibration time: | 2 min | A: 100% | B: 0% |
| Start method: | 0–2 min | A: 100% | B: 0% |
| Gradient: | 2–15 min | A: 100–0% | B: 0–100% |
| 15–17 min | A: 0% | B: 100% | |
| Gradient: | 17–19 min | A: 0–100% | B: 100–0% |
| 19–21 min | A: 100% | B: 0% |
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