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Protein sequence alignment of the [FeFe]-hydrogenases of C. pasteurianum W5 and C. acetobutylicum. Residues ligating FeS clusters are indicated with A, B, C, or 2 to denote the cluster they ligate; A – (FS4A), B – FS4B, C – FS4C, and 2 – FS2. The H-cluster coordinating motifs are designated by brackets showing L1, L2, and L3 (Vignais and Billoud, 2007).
