Representative water-scaled estimated GABA+ levels in non-, early and late exposed subjects, before and after MPH challenge. Data are represented as mean, error bars represent the standard error of the mean. *p < 0.05. Before the MPH challenge, the EST and LST subjects differed significantly (p = 0.04), whereas only the EST subjects showed increased estimated GABA+ levels after the MPH challenge (p = 0.01).