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. 2016 Jun 3;19(4):418–424. doi: 10.4103/1008-682X.181816

Figure 2.

Figure 2

Inhibitory effects of the chloride channel blockers NPPB and DIDS (both at 100 μmol l−1) on the motility of spermatozoa from the normozoospermic and asthenozoospermic semen samples in hypotonic BWW290. The inhibitory effects of NPPB and DIDS on the (a) progressive motility (PR), (b) straight-line velocity (VSL), (c) curved-line velocity (VCL) and (d) average path velocity (VAP), respectively, when incubated for 30 min. Data are shown as the mean ± s.e. (semen samples, n from 8 to 22). **P < 0.01 asthenozoospermia vs normozoospermia. s.e.: standard error; DIDS: 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid; NPPB: 5-nitro-2-(3-phenylpropylamino) benzoic acid; BWW: Biggers, Whitten and Whittingham.