Table 2. Primers used in the real-time PCR.
Genes1 | Primer sequence2 (5′-3′) | Accession no. |
---|---|---|
β-Actin | F: GAGAAATTGTGCGTGACATCA | L08165 |
R: CCTGAACCTCTCATTGCCA | ||
Mucin-2 | F: TTCATGATGCCTGCTCTTGTG | XM_421035 |
R: CCTGAGCCTTGGTACATTCTTGT | ||
Claudin-1 | F: CATACTCCTGGGTCTGGTTGGT | AY750897.1 |
R: GACAGCCATCCGCATCTTCT | ||
SGLT1 | F: GATGTGCGGATACCTGAAGC | AJ236903 |
R: AGGGATGCCAACATGACTGA | ||
PepT1 | F: TACGCATACTGTCACCATCA | AY029615 |
R: TCCTGAGAACGGACTGTAAT | ||
L-FABP | F: GAAGGGTAAGGACATCAA | NM_204192 |
R: TCGGTCACGGATTTCAGC |
1SGLT1, sodium glucose cotransporter 1; PepT1, H+-dependent peptide transporter 1
L-FABP, liver fatty acid-binding protein.
2 F, forward; R, reverse.