Fig 7. Effect of a single 5 ns pulse at different E-field amplitudes on maximal Na⁺ conductance.

The protocol (inset) was the same as that for generating steady-state activation of I_Na. Peak I_Na was converted to conductance as already described and relative conductance obtained by normalizing the conductance both before and after the pulse to the maximum conductance before the pulse. The data were fitted to a Boltzmann function (see Methods) and expressed as the mean ± SE. (A) Pulse applied at an E-field of 5 MV/m (n = 6) and (B) at 8 MV/m (n = 5).