Table 2. Epitope localization by mAb competitive binding assays.

mAb a	SylH3	24B11	MH3	8A1	8B3	LF1	LC5
SylH3	97	<10	<10	<10	85	<10	<10
24B11	<10	96	91	55	92	95	<10
MH3	<10	37	89	<10	43	34	<10
8A1	<10	35	<10	81	45	57	<10
8B3	87	79	86	21	92	93	<10
LF1	<10	50	56	26	62	95	<10
LC5	<10	<10	<10	<10	<10	<10	79

^a, competitive sandwich ELISAs were conducted as described in the Materials and Methods.

The capture mAbs (1 μg/ml) listed in the top row were coated on the 96 well microtiter plates, while the competitor mAbs (10 μg/ml) listed in the far left column were mixed with biotin-tagged ricin (equivalent to the EC₉₀ for each coated mAb) in solution then applied to the microtiter plates. The numbers are the % inhibition of ricin capture, as defined in the Materials and Methods.