Table 1. Primer pairs for real-time PCR.
| Gene name | Sequences of two way primers | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|
| GAPDH |
F:5’CACTGAGCAAGAGAGGCCCTAT3’ R:5’GCAGCGAACTTTATTGATGGTATT3’ |
60 | 144 |
| Fas |
F:5’ ATCTGGGCTGTCCTGCCTCTG3’ R:5’ TCAGTTTCACGAACCCGCCTC3’ |
60 | 113 |
| Traf2 |
F:5’ CAGAAAGCGTCAGGAAGCCGTAG3’ R:5’ CAGTGCCGTCGCCATTCAAGT 3’ |
60 | 119 |
| Il1b |
F:5’ CTTCAGGCAGGCAGTATCACTC3’ R:5’ GCAGTTGTCTAATGGGAACGTC3’ |
60 | 194 |
| Pik3r1 |
F:5’ TCTACCCAGTGTCCAAATACCAG3’ R:5’ TAAATGCTTCGATAGCCGTTC3’ |
60 | 189 |
| Pik3r5 |
F:5’ ATCGGATTTCAGGGAAGGTGG3’ R:5’ GGAGTGGAGATGCTTGGCTCA3’ |
60 | 174 |
| Ppp3cc |
F:5' TTCCTCTTGCTGCCCTCTTAA3’ R:5’ TTCTCGCTGCCGTAGTCCTCT3’ |
60 | 175 |
| Pik3cd |
F:5' CATCAGTGGCTCTGCGGTTTG3’ R:5’ ATCTCCTTGGTTTGGGGCTTG 3’ |
60 | 169 |
| Csf2rb2 |
F:5' GGACATAGAGTTTGAGGTGGC3’ R:5’ AGCATAGATGCTGTTGGGTAG3’ |
60 | 127 |
| Map3k14 |
F:5' AAGACCGAGCCCTTTACTACC3’ R:5’TCAGCTTTGACATCGCCATGC3’ |
60 | 91 |