Table 1. Antibodies used in this study.
Antigen | Source | Antibody type | Application | Dilution |
---|---|---|---|---|
Integrin α6 | Abcam 124924 | R monoclonal | WB | 1:2000 |
Integrin β1 | Abcam 134179 | R monoclonal | WB | 1:500 |
p63α | Santa Cruz 8344 | R polyclonal | WB | 1:1000 |
CD44 | Cell Signaling 5640 | M monoclonal | WB | 1:1000 |
β-catenin | Cell Signaling 2698 | M monoclonal | WB | 1:1000 |
β-catenin | Cell Signaling 2677 | M monoclonal | IF | 1:300 |
GAPDH | Cell Signaling 2118 | R monoclonal | WB | 1:1000 |
Non-phospho-(active) β-catenin (S33, S37, T42) | Cell Signaling 8814 | R monoclonal | WB | 1:1000 |
p-β-catenin (S45) | Cell Signaling 9564 | R polyclonal | WB | 1:1000 |
p-GSK-3β (S9) | Cell Signaling 9322 | R monoclonal | WB | 1:1000 |
GSK-3β | Cell Signaling 9315 | R monoclonal | WB; IP | 1:1000; 1:50 |
GSK-3β | Cell Signaling 9832 | M monoclonal | WB; IP | 1:1000; 1:50 |
PR55α | Cell Signaling 2290 | R monoclonal | WB; IP | 1:1000; 1:25 |
PR55α | Cell Signaling 5689 | M monoclonal | WB | 1:1000 |
PP2A C subunit | Cell Signaling 2038 | R polyclonal | WB | 1:1000 |
PP2A C subunit | Abcam 66597 | M monoclonal | IP | 1:75 |
p-mTOR (S2448) | Cell Signaling 5536 | R monoclonal | WB | 1:1000 |
p-p70 S6K (T389) | Cell Signaling 9205 | R polyclonal | WB | 1:1000 |
p-Rictor (T1135) | Cell Signaling 3806 | R monoclonal | WB | 1:1000 |
p-Akt (S473) | Cell Signaling 4060 | R monoclonal | WB | 1:2000 |
p-Akt (T308) | Cell Signaling 4056 | R monoclonal | WB | 1:1000 |
Akt | Cell Signaling 4691 | R monoclonal | WB | 1:1000 |
p-PDK1 (S241) | Cell Signaling 3438 | R monoclonal | WB | 1:1000 |
Lamins A+ C | Cell Signaling 4777 | M monoclonal | WB | 1:2000 |
p-GSK-3α (S21) | Cell Signaling 9316 | R monoclonal | WB | 1:1000 |
GSK-3α | Cell Signaling 4337 | R monoclonal | WB | 1:1000 |
p-β-catenin (S675) | Cell Signaling 4176 | R monoclonal | WB | 1:1000 |
p-LRP6 (S1490) | Cell Signaling 2568 | R ployclonal | WB | 1:1000 |
LRP6 | Cell Signaling 3395 | R monoclonal | WB | 1:1000 |
E-cadherin | Abcam 40772 | R monoclonal | IF | 1:100 |
R: rabbit; M: mouse