Fig 4. In vivo mitochondria function is impaired after exposure to a single Cy dose.

(A) Maximal mitochondrial ATP production (ATPmax) was reduced in the hindlimb skeletal muscles of Cy treated mice compared 1 day and 6 weeks after treatment. In the 1 day dataset one data point that was outside the typical range of values and that failed the outlier test was excluded from the analysis. Excluding this point makes data significantly depressed at P = 0.036. (B) PCr/ATP ratio was calculated from mean PCr and ATP concentrations (S1 Table). Cy treatment led to a reduced PCr/ATP ratio at both timepoints. (C) There was no effect of Cy treatment on coupling of oxidative phosphorylation (P/O). Data presented as box plot showing min, median, and max data point. (N = 5 for baseline and 1 day timepoints, N = 8 for 6 week timepoint) (NS, not significant).