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. 2017 Jun 23;13(6):e1006857. doi: 10.1371/journal.pgen.1006857

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© 2017 Choi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Maximum likelihood phylogeny of (A) β-tubulins, (B) ligand-gated cation channels, (C) ligand-gated anion channels, and (D) ATP-binding cassette transporters in Teladorsagia circumcincta. Unsupported nodes (bootstrap support less than 50%) were collapsed to polytomy. The shading on the trees highlights monophyletic groups. Caenorhabditis elegans (Cel) and Haemonchus contortus (Hco) homologs were included to help resolve the phylogeny. Tubulin from Saccharomyces cerevisiae (Sce-TUB-2) served as an outgroup. Gene-wise fixation index (F_ST) and copy number variation (CNV) between the RS³ and S_inbred populations of T. circumcincta were represented as a heatmap. Non-synonymous coding variants identified in the F_ST outlier genes (z-socre > 4.5) were reported and loci with >50% allele frequency differences were indicated in bold. De novo assembly [101] of β-tubulin isotype-1 (TELCIR_01271) from the RS³ strain revealed haplotypes harboring E198L (GAa/TTa) and F200Y (tTc/tAc) variants.