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. Author manuscript; available in PMC: 2017 Jul 12.
Published in final edited form as: Chem Res Toxicol. 2012 Feb 6;25(2):326–336. doi: 10.1021/tx200376e

Figure 3. Dependence of DNA-protein crosslinking on oligonucleotide structure and oxidant type.

Figure 3

(A) The degree of oxidative crosslinking with a single stranded DNA, SS, is compared with that of double stranded, DS, DNA. The two DNA structures have a comparable tendency to form DNA-protein crosslinks. (B) Four different oxidant systems were used to generate crosslinks at equimolar concentrations of protein to DNA. Fe(II)-H2O2, Cu(II)-H2O2, riboflavin and rose bengal oxidation systems all generated high yields of crosslink despite the divergent oxidation mechanisms. Error bars are standard deviations with n=3. (C) Rose bengal induced crosslinks occur rapidly and linearly increase with irradiation time within the first twenty minutes.