Fig 3. Inhibitory effect of ROS and ROS generator on human DGAT2 catalytic activity.

Membrane extracts from human DGAT2-overexpressing Sf9 insect cells were treated with indicated concentrations of H₂O₂ (A) or β-lapachone (B) in the presence or absence of 20 mM DTT. Human DGAT2 activity was measured by using the conventional extraction-based in vitro assays which are described in detail in the Materials and Methods section. The activities of membrane extracts treated with PBS (instead of H₂O₂) or DMSO (instead of β-lapachone) in the absence of DTT were defined as 100%. The mean values and standard deviations were determined from four independent experiments.