Fig 5. Multimeric complex of human DGAT2 formed by H₂O₂-induced disulfide crosslinking in human cells.

Huh-7 cells were transfected with plasmid overexpressing human DGAT2 for 47 hours and further incubated with indicated concentrations of H₂O₂ for 1 hour. Cell extracts were harvested in a way described in Materials and Methods section and subjected to Western blot analysis using anti-DGAT2 antibody (A). The amount of monomeric human DGAT2 proteins presented as redDGAT2 in (A) was quantified and the amount of relative redDGAT2 protein was calculated by setting the values from samples treated with PBS to 100% (B). The mean values and standard deviations were determined from three independent experiments. Asterisks indicate non-specific bands.