Fig 4. TbCDC34 is necessary for infection progression in mice.

Parasitemia was monitored in peripheral blood from one to four days after intraperitoneal inoculation of parental BSF T. brucei cell line 90–13 (left) or TbCDC34-RNAi BSF T. brucei cells (right). Dashed vertical lines indicate the times at which doxycycline was added to the drinking water of the selected animals. Application of doxycycline to the TbCDC34-RNAi cell line (+Dox, right panel) resulted in clearance of trypanosomes from mice. This did not occur in mice not given doxycycline (-Dox, right panel). These results are representative of at least three experiments. Crosses (✝) indicate that the mice reached a humane end point within 24 h of the last recorded parasitemias. Error bars represent the ± SEM of 5 biological replicates.