Table 1. Leptospira detection and identification by host species.
The prevalence of renal infection by Leptospira in farm pigs and rodents is based on RT-qPCR detection results. Infecting Leptospira species were determined by PCR, targeting either secY, rrs (16S) or LipL32 gene in positive samples. The number of successful amplification for secY is provided. Based on the observed diversity within the secY gene, MLST amplifications were attempted on a subset of positive samples; the number of successful complete MLST on seven (or six) loci is given.
| Source | Leptospira prevalence (%) | Identification success (%) | Infecting Leptospira | secY amplification | 7-loci (or 6a) MLST |
|---|---|---|---|---|---|
| Pig | 48/181 (26.5) | 30/48 (62.5) | Li (n = 30), L* (n = 10) | 30 | 4/14 (5) |
| RR | 3/28 (10.7) | 2/3 (66.7) | Li (n = 2) | 2 | 0/2 (1) |
| RN | 19/79 (24.1) | 13/19 (68.4) | Li (n = 8), Lb (n = 5) | 12 | 3/12 (6) |
| RE | 1/6 (16.7) | 1/1 (100) | Lb (n = 1) | 1 | 1/1 (1) |
| All rats | 23/113 (20.4) | 16/23 (69.6) | Li (n = 10), Lb (n = 6) | 15 | 4/15 (8) |
| Dog | - | 2/4 (50) | Li (n = 2) | 2 | 0 |
| Human | - | 39/51 (76.5) | Li (n = 37), Lb (n = 2) | 33 | 4/9 (4) |
RR: Rattus rattus;
RN: Rattus norvegicus;
RE: Rattus exulans.
Li: Leptospira interrogans;
Lb: Leptospira borgpetersenii;
L*: undetermined species closely related to Leptospira mayottensis and Leptospira alexanderi.
a Amplification and sequencing success for the 6-loci MLST scheme excluding the locus caiB.