Fig 2. Comparison of lipid recovery from fresh, intact silks with different extraction solvents.

A. Total extracellular surface lipid recovery (including hydrocarbons, fatty acids and aldehydes) from fresh, emerged silks of inbred B73 extracted for four minutes with the specified solvents: hexanes, hexanes:diethyl ether (95:5 and 90:10), chloroform:hexanes (1:1) and chloroform. B. Relative abundances (%) of observed extracellular surface lipid classes. C. Relative abundances (%) of saturated (alkanes) and unsaturated (alkenes) relative to total hydrocarbon recovery. D. Relative abundances (%) of recovered unsaturated 18-carbon fatty acids relative to total fatty acid recovery. Different lowercase letters above or within data-bars for a specific lipid class or constituent denote statistically significant differences in recovery with different solvents, at p<0.05, according to a Tukey’s HSD test. In panel A, uppercase letters denote statistically significant differences among total extracellular surface lipid recoveries. A minimum of five replicates were used per solvent, with an N = 29. Averages ± SE are reported.