Fig 3. Comparison of lipid recovery from lyophilized, powderized silks extracted with different solvents.

A. Total extracted lipid recovery (including hydrocarbons, fatty acids, aldehydes) from lyophilized, powderized emerged silks of inbred B73 extracted for ten minutes with the specified solvents: hexanes, hexanes:diethyl ether (95:5 and 90:10), chloroform:hexanes (1:1) and chloroform. B. Relative abundances of the observed lipid classes, hydrocarbons and fatty acids. Aldehydes were not detected above the limit of quantitation in this experiment. C. Relative abundances (%) of saturated (alkanes) and unsaturated (alkenes) relative to total hydrocarbon recovery. D. Relative abundances (%) of unsaturated 18-carbon fatty acids relative to total fatty acid recovery. Different lowercase letters above or within data-bars for a specific lipid class or constituent denote statistically significant differences in recovery with different solvents, at p<0.05, according to a Tukey’s HSD test. In panel A, uppercase letters denote statistically significant differences among total extracellular surface lipid recoveries. A minimum of four replicates were used per solvent, with an N = 28. Averages ± SE are reported.