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. 2017 Jul 12;12(7):e0180225. doi: 10.1371/journal.pone.0180225

Fig 4. BRG1 knockdown impairs colony formation and modulates cyclin family.

Fig 4

(A,B) Colony formation assay was performed to analyse proliferation of both human HCC cell lines HepG2 and HuH7. Colony formation was reduced significantly in both cell lines, HepG2 and HuH7, n = 3. (C) Analysis of mRNA expression by qRT-PCR showed a significant down-regulation of cyclinB and cyclinE expression in BRG1-suppressed HepG2 cells at the time point of 40h after transfection, n = 4. (D) HuH7 cells showed a trend towards lower expression though this was not significant, n = 4. Negative control was standardised as 1.