Table 1. Primers used in this study.
Cloning primers | ||
Primer names | Oligonucleotide sequence (5’ to 3’) | Fragment names (bp) |
Tetur-VATP-F-A | CAGTTCTCCGAACCGGTAAA | A (214) |
Tetur-VATP-R-A | CCACCGGTAATGTGACTACCA | |
Tetur-VATP-F-B | CCGTGATATGGGTTACCATG | B (416) |
Tetur-VATP-R-B | GAAGAGGTACGAAATCTGGG | |
Tetur-sc12-F | GCCCTCTCCTGGTTGTAAACTT | Control, NC (382) |
Tetur-sc12-R | CGACCCCATCAGGCTATTGA | |
Spider mite RT-qPCR assay primers | ||
Primer names | Oligonucleotide sequence (5’ to 3’) | Primer efficiency |
RP49 (tetur18g03590) F | CTTCAAGCGGCATCAGAGC | 97.6% |
RP49 (tetur18g03590) R | CGCATCTGACCCTTGAACTTC | |
VATPase qPCR F | GGGTACCATCACATTCCTCG | 104.1% |
VATPase qPCR R | AATCGGTCTGGTTTGACGAAC | |
Transgenic plant RT-qPCR assay primers | ||
Primer names | Oligonucleotide sequence (5’ to 3’) | Primer efficiency |
PEX4 (AT5G25760) F | GCTCTTATCAAAGGACCTTCGG | 99.2% |
PEX4 (AT5G25760) R | CGAACTTGAGGAGGTTGCAAAG | |
VATPase qPCR F | GGGTACCATCACATTCCTCG | 104.1% |
VATPase qPCR R | AATCGGTCTGGTTTGACGAAC |