Skip to main content
. 2017 Jul 12;12(7):e0180654. doi: 10.1371/journal.pone.0180654

Table 1. Primers used in this study.

Cloning primers
Primer names Oligonucleotide sequence (5’ to 3’) Fragment names (bp)
Tetur-VATP-F-A CAGTTCTCCGAACCGGTAAA A (214)
Tetur-VATP-R-A CCACCGGTAATGTGACTACCA
Tetur-VATP-F-B CCGTGATATGGGTTACCATG B (416)
Tetur-VATP-R-B GAAGAGGTACGAAATCTGGG
Tetur-sc12-F GCCCTCTCCTGGTTGTAAACTT Control, NC (382)
Tetur-sc12-R CGACCCCATCAGGCTATTGA
Spider mite RT-qPCR assay primers
Primer names Oligonucleotide sequence (5’ to 3’) Primer efficiency
RP49 (tetur18g03590) F CTTCAAGCGGCATCAGAGC 97.6%
RP49 (tetur18g03590) R CGCATCTGACCCTTGAACTTC
VATPase qPCR F GGGTACCATCACATTCCTCG 104.1%
VATPase qPCR R AATCGGTCTGGTTTGACGAAC
Transgenic plant RT-qPCR assay primers
Primer names Oligonucleotide sequence (5’ to 3’) Primer efficiency
PEX4 (AT5G25760) F GCTCTTATCAAAGGACCTTCGG 99.2%
PEX4 (AT5G25760) R CGAACTTGAGGAGGTTGCAAAG
VATPase qPCR F GGGTACCATCACATTCCTCG 104.1%
VATPase qPCR R AATCGGTCTGGTTTGACGAAC