Fig. 10.

Rutin as an inhibitor of wild-type and CxxC mutant PDIs. (A) Following a 15 min incubation of 75 nM PDI_red or 150 nM mutant PDI_red with 0 (black), 1 (gray) or 10 (gray stripe) μM rutin, 714 nM BD-SS was added and the increase in fluorescence was monitored. Percent fluorescence after 30 s was normalized to PDI_red without inhibitor. (B) Multiple turnover assays were completed including 5 mM in GSH in the preincubation as above.