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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 Apr 30;108:741–749. doi: 10.1016/j.freeradbiomed.2017.04.367

Fig. 3.

Fig. 3

Assay reagents and depictions of multiple turnover and reoxidation assays. (A) Dieosin-GSSG (Di-E-GSSG) and BODIPY FL L-cystine (BD-SS) can be used as “turn-on” fluorophores to measure the reductase activity of PDI. (B) Multiple turnover assays are sustained by the inclusion of an exogenous reductant (depicted by R-SH). (C) The reoxidation of reduced PDI by a fluorogenic disulfide in the absence of exogenous reductant.