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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 Apr 30;108:741–749. doi: 10.1016/j.freeradbiomed.2017.04.367

Fig. 5.

Fig. 5

Effect of MNS on PDI activity in multiple turnover and reoxidation assays. (A) Activity of 75 nM oxidized PDI with 0 (——), 10 (– – –) or 50 (. . .) μM MNS in the presence of 5 mM GSH (corrected for non-enzymatic background, see Materials and Methods). (B) The development of fluorescence upon mixing 714 nM BD-SS to 75 nM PDIred preincubated for 15 min with 0 (——), 10 (– – –) or 50 μM (. . .) MNS. Curves represent the average of traces recorded in triplicate.