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Titration of reduced RNase with MNS. MNS, 40 μM, was mixed with 10–200 μM RNase thiols and the absorbance at 378 nm was followed with time. The reaction was rapid, with a half life of less than 2 s at each concentration. The change in absorbance at 378 nm was recorded 30 s after mixing (see Materials and Methods). Titrations were performed in triplicate. The solid line represents a Kd of 0.1 μM.
