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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 Apr 30;108:741–749. doi: 10.1016/j.freeradbiomed.2017.04.367

Fig. 8.

Fig. 8

Effects of APAO on PDI activity. (A) Schematic of APAO interaction with thiols. (B) Left: following incubation of 75 nM PDIred with 0 (black), 50 (gray), and 100 (gray stripe) μM APAO for 15 min, assays were initiated by BD-SS addition. Fluorescence after 30 sec was plotted for triplicate experiments normalized to the corresponding value in the absence of APAO. Right: multiple turnover assay incubating PDIox with 5 mM GSH and 0 (black), 50 (gray), or 100 (gray stripe) μM APAO. Percent fluorescence was plotted for triplicate experiments 30 s after BD-SS injection as before.