Figure 3.

Effect of pannexin-1 gene knockdown on TLR2- and TLR4-induced IL-1β release from human monocytes. THP-1 cells were transfected with 100 µM of pannexin-1 siRNA or scrambled siRNA using Amaxa Nucleofector transfection system. A, B) After 48 h, gene expression was measured by real-time quantitative PCR (A) and protein levels were measured by Western blot analysis (B). C, D) Transfected THP-1 monocytes were stimulated with Pam₃CSK4 (0.01 to 1 μg/ml) (C) and LPS (0.01 to 1 μg/ml) (D). PCR transfection data are representative of 3 separate transfections; Western blot analysis is representative of these transfections. Stimulation data represent means ± sem of 9 replicates. *P ≤ 0.05 as assessed by 1-way ANOVA followed by Bonferroni post hoc test.