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Subtomogram averaging analysis of SA orientation on the TMV surface. (A) Averaged SA density (transparent gray) and the human SA crystal structure33 (PDB: 1AO6, gray ribbon) positioned with respect to the TMV cylinder (blue) at five locations along a single SA-TMV nanoparticle. These locations are shown both with (top) and without (bottom) the cryo-ET density (red). (B) SA crystal structure (PDB: 1AO6) with the C-alpha atoms of solvent-exposed lysine residues depicted as blue spheres.
