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. 2017 Jul 5;20(1):173–187. doi: 10.1016/j.celrep.2017.06.027

Figure 1.

Figure 1

Genetic Ablation of LIMD1 Switches Dependency upon Argonaute Species for miRNA Silencing

(A) psiCHECK-2-miR-99/100 luciferase reporter (NT, non-targeting reporter; T, targeting reporter; mean ± SEM; n = 4 treated with indicated siRNAs (SCR, non-targeting control).

(B and C) (B) psiCHECK-2-miR99/100 reporter in CRISPR-Cas9 gene-edited HeLa cells with Cas9 alone control (LIMD1+/+) or (C) LIMD1 knockout (LIMD1−/−) cells, treated with the indicated siRNAs.

(D and E) (D) psiCHECK-2-FGFR3 (endogenous mutant [MUT] or wild-type [WT] 3′ UTR) reporter in CRISPR-Cas9 gene-edited HeLa cells with Cas9 alone control (LIMD1+/+) or (E) LIMD1 knockout (LIMD1−/−) cells, treated with the indicated siRNAs.