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. 2017 Jul 1;23:3209–3216. doi: 10.12659/MSM.902441

Figure 6.

Figure 6

Effects of Astragaloside and Baicalin on IL-8, IL-1β, and TNF-α expression and the relationship with the MAPK/ERK pathway. After the addition of U0126 and MAPK/ERK pathway inhibitor, we used ELISA, RT-PCR, and Western blot to detect the levels of inflammatory cytokines to clarify whether the effects involved the MAPK/ERK pathway. (A) IL-8, IL-1β, and TNF-α expression of MSCs supernatant with ELISA. (B) IL-8, IL-1β, and TNF-α expression of MSCs supernatant with RT-PCR. (C) IL-8, IL-1β, and TNF-α expression of MSCs supernatant with Western blot.