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Transfection efficiency (percentage of fluorescent cells) obtained with different polymer/pDNA ratios (1:1 and 2:1) in DMEM without FBS
| Polymer/pDNA ratio (w/w) | 1:1 | 2:1 | |||
|---|---|---|---|---|---|
| pDNA (μg) | 2 | 1 | 0.50 | 1 | 0.50 |
| PEI 22K | 2.5 | 0.7 | 0.8 | 21.2 | 2.6 |
| PEI 25K | 32.5 | 13.5 | 1.2 | 38.4 | 12.2 |
| PEI 87K | 1.1 | 0.5 | 0.6 | 24.0 | 2.7 |
| PEI 217K | 13.0 | 7.3 | 0.6 | 22.1 | 15.1 |
| PAH | 16.2 | 2.5 | 2.3 | 7.9 | 2.3 |
