Table 4.

Frequency of binucleated cells with micronucleus, Nuclear Division Index (NDI) and percentage of reduction in mutagenicity (%Red) obtained in CBMN assay performed with digoxin associated with MMC in different protocols of treatment on CHO-K1 cell line

Treatments	Protocols
	Pre-complexation			Pre-treatment			Simultaneous treatment			Post-treatment
	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)
PBS	6.3 ± 1.2*	1.5 ± 0.01		7.3 ± 0.9*	1.5 ± 0.01		7.0 ± 0.8*	1.5 ± 0.01		11.0 ± 0.8*	1.5 ± 0.01
MMC	30.7 ± 1.7	1.5 ± 0.01		30.7 ± 1.7	1.5 ± 0.01		30.0 ± 1.6	1.5 ± 0.01		29.7 ± 1.2	1.5 ± 0.01
0.7 + MMC	29.0 ± 0.8	1.5 ± 0.01	–	28.0 ± 0.8	1.5 ± 0.01	–	26.7 ± 1.2	1.5 ± 0.01	–	30.3 ± 1.2	1.5 ± 0.01	–
1.5 + MMC	24.7 ± 1.2*	1.5 ± 0.01	24.4	21.3 ± 1.2*	1.5 ± 0.01	40.2	23.3 ± 0.47*	1.5 ± 0.01	28.8	28.7 ± 0.9	1.5 ± 0.01	–
3.0 + MMC	17.7 ± 1.2*	1.5 ± 0.01	53.1	18.0 ± 0.8*	1.5 ± 0.01	54.3	15.0 ± 0.82*	1.5 ± 0.01	65.2	27.3 ± 0.9	1.5 ± 0.01	–

MNBN binucleated cells with micronucleus, $\overline{x}\pm SD$ mean ± standard deviation, MMC Mitomycin C (4.8 µM)

* Statistically different from group treated with mutagen MMC (p < 0.05)

^#Statistically different from negative control (PBS) (p < 0.05)