Table 5.

Frequency of binucleated cells with micronucleus, Nuclear Division Index (NDI) and percentage of reduction in mutagenicity (%Red) obtained in CBMN assay performed with digoxin associated with MMC in different protocols of treatment on the HeLa cell line

Treatments	Protocols
	Pre-complexation			Pre-treatment			Simultaneous treatment			Post-treatment
	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)	MNBN ($\overline{\text{x}}\pm \text{SD})$	NDI ($\overline{\text{x}}\pm \text{SD})$	%Red (%)
PBS	8.0 ± 0.8*	1.5 ± 0.01		8.7 ± 1.2*	1.5 ± 0.02		6.7 ± 1.2*	1.5 ± 0.03		8.0 ± 1.6*	1.4 ± 0.01
MMC	39.7 ± 1.2	1.4 ± 0.01#		36.3 ± 1.2	1.4 ± 0.01#		39.0 ± 1.6	1.3 ± 0.01#		35.0 ± 1.4	1.4 ± 0.00#
0.7 + MMC	37.3 ± 0.9	1.3 ± 0.01#	–	38.0 ± 2.2	1.2 ± 0.03#	–	35.0 ± 1.4	1.2 ± 0.02#	–	34.7 ± 1.7	1.4 ± 0.01	–
1.5 + MMC	38.7 ± 1.2	1.4 ± 0.01#	–	27.7 ± 1.2*	1.4 ± 0.01	31.3	38.3 ± 1.2	1.5 ± 0.02	–	31.3 ± 2.0	1.4 ± 0.01	–
3.0 + MMC	23.0 ± 1.6*	1.5 ± 0.01	52.6	31.7 ± 1.7*	1.4 ± 0.01	34.0	22.7 ± 1.2*	1.5 ± 0.02	50.3	27.3 ± 0.9	1.4 ± 0.01	–

MNBN binucleated cells with micronucleus, $\overline{x}\pm SD$ mean ± standard deviation, MMC Mitomycin C (4.8 µM)

* Statistically different from group treated with mutagen MMC (p < 0.05)

^#Statistically different from negative control (PBS) (p < 0.05)