Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 12;7:5223. doi: 10.1038/s41598-017-05273-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparison of biofilms measured with inverted confocal laser scanning microscopy (CLSM) and with crystal violet staining. (A) Schematic visualization of biofilm localization in a microtiter plate well. (B) Example of the biofilm stained with crystal violet in an E-plate 96 (ACEA, USA). (C) Biofilm biomass determined with crystal violet staining in a half-area, µclear microtiter plate (n = 12) (Greiner Bio-One). The crystal violet background of the medium was subtracted. (D) Easy 3D projections of biofilm structures stained with the BacLight Viability Kit. (E) Biofilm biovolume determined from biofilm structures as depicted in Fig. 1D with a customized solution of the image analysis software Definiens (n = 6). Red bars: PA14 Wild type; olive bars: pqsA::Tn mutant; light green bars: pelA::Tn mutant. n indicates the number of technical replicates.