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. 2017 Jul 12;7:5223. doi: 10.1038/s41598-017-05273-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Changes in the standardized single-frequency impedance (expressed as cell indices) and (B) crystal violet staining measurements of pellicle biofilm over 72 h, during biofilm growth in LB culture medium of PA14 wt, pqsA::Tn mutant, and pelA::Tn mutant (n = 24 for each mutant) and LB Medium (n = 8). The PA14 wild type and pqsA::Tn are biofilm formers; pelA::Tn does not form biofilm. n indicates the number of technical replicates. The period of linear decline of the cell index is highlighted by a yellow bar.