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. 2017 Jul 12;7:5238. doi: 10.1038/s41598-017-05566-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Efficient purification of HIV-1-infected and bystander MØ. (A) The murine HSA reporter gene was transcriptionally fused to the virus-encoded Nef gene to engineer the infectious molecular clone NL/Bal-HSA. MØ are first infected with NL/Bal-HSA reporter virus followed by an HSA-based immunomagnetic sorting to isolate and purify HSApos and HSAneg MØ. (B) Surface staining of the HSA epitope correlates with Gag expression (KC57 antibody) in flow cytometry analyses at 6 dpi. Data shown are representative of two replicate experiments. (C) Surface staining of the HSA epitope correlates with CD4 depletion at 6 dpi. Data are from a representative experiment of two replicate experiments. (D) HSApos MØ are efficiently enriched by the HSA-based immunomagnetic sorting process independently of the initial HIV-1 infection rate at 36 hpi or 6 dpi. MØ preparations from 20 distinct donors were used for these studies.