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. 2017 Jul 12;7:5238. doi: 10.1038/s41598-017-05566-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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siRNA screen reveals new regulators of HIV-1 replication in MØ. (A) Vector maps of fully competent infectious molecular clones NL/Bal-eGFP and NL/Bal-FLuc (left panel) and schematic representation of the experimental protocol used in the siRNA screen (right panel). (B) siRNA sequences directed against fifty different host genes selected from RNAseq analyses allowed identification of interesting candidates for new regulators of HIV-1 replication in MØ (orange dots). siRNAs directed against SAMHD1 were used as controls for restriction factors (red dots) and siRNAs against CD4 were used as controls for susceptibility factors (blue dots). Data are presented as the mean of six independent experiments. (C) Transcriptional expression of MDM2, LOXL3, ATM and APOBEC3B genes was monitored following siRNA delivery. Data are presented as the mean ± SD. Asterisks denote statistically significant data (**p < 0.01; ***p < 0.001; ****p < 0.0001; NS: not significant).