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. 2017 Jul 12;7:5217. doi: 10.1038/s41598-017-05567-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of bone marrow cells with α-toxin decreases TER119+ erythroblasts. Bone marrow cells were isolated from mice, and flow cytometry analysis was performed using a Guava easyCyte. (A,B) A total of 5 × 10⁶ bone marrow cells were cultured for 24 hours in the presence of the indicated concentration of α-toxin (Wild-type) or a variant α-toxin (H148G). The frequency (A) and absolute number of TER119⁺ cells per culture well (B) are shown. (C,D) Bone marrow cells were cultured for 3, 6, or 24 hours in the presence of 10 ng/ml α-toxin (Wild-type) or a variant α-toxin (H148G), and the frequency (C) and absolute number of TER119⁺ cells per culture well (D) are shown. One-way ANOVA was employed to assess statistical significance. Values are mean ± standard deviation. *P < 0.05; **P < 0.01; n.s., not significant.