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. 2017 Jul 12;7:5217. doi: 10.1038/s41598-017-05567-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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α-Toxin preferentially eradicates mature erythroblasts. (A,B) A total of 5 × 10⁶ bone marrow cells were cultured for 24 hours in the presence of 10 ng/ml α-toxin (Wild-type) or a variant α-toxin (H148G), and flow cytometry analysis was performed using a Guava easyCyte. (A) A representative flow cytometry profile is shown. (B) The absolute number of CD71⁺TER119⁻, CD71⁺TER119⁺, and CD71⁻TER119⁺ cells per culture well was determined. (C,D) Bone marrow cells were treated with the indicated concentration of α-toxin (Wild-type) or a variant α-toxin (H148G), and the cells were plated in Methocult 3334. CFU-E (C) and BFU-E (D) were determined. One-way ANOVA was employed to assess statistical significance. Values are mean ± standard deviation. **P < 0.01; n.s., not significant.