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. 2017 Jul 12;7:5217. doi: 10.1038/s41598-017-05567-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The in vivo effect of α-toxin on erythropoiesis. Mice were injected intraperitoneally with 80 ng of purified α-toxin (α-Toxin) or PBS as a control (Control). BMCs and peripheral blood were isolated 24 hours after the injection. (A) Bone marrow cellularity was determined. (B–E) Flow cytometry analysis of BMCs was performed using specific antibodies against CD71 and TER119. A representative flow cytometry profile (B), the frequency of CD71⁺TER119⁻ cells (C), CD71⁺TER119⁺ cells (D), and CD71⁻TER119⁺ cells (E) are shown. (F) Plasma EPO level was measured by ELISA. Two-tailed Student’s t-test was employed to assess statistical significance. Values are mean ± standard error. **P < 0.01; ***P < 0.001; n.s., not significant.