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. 2017 Jul 12;7:5249. doi: 10.1038/s41598-017-05489-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TSPAN1 increases prostate cancer cell migration and induces the expression of Slug and ARF6. (A,C) Validation of TSPAN1 overexpression in DU145 and PC3 cells by real-time PCR and western blot. (B,D) Migration assays for DU145 and PC3 cells overexpressing TSPAN1. (E,F) Western blot analysis of Slug and ARF6 protein expression in DU145 and PC3 cells overexpressing TSPAN1. Please note: cropped western blots are displayed in the figure, but full length blots are included in the Supplementary Information file.