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. 2017 Jul 12;7:5249. doi: 10.1038/s41598-017-05489-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TSPAN1 is essential for prostate cancer cell viability. Depletion of TSPAN1 in (A) LNCaP and (B) CWR22 RV1 cells by transient transfection with siRNA. Protein depletion was carried out using two different siRNAs in cells grown in full media. 96 hours after transfection knockdown of TSPAN1 was confirmed by real-time PCR and western blot and the relative number of live cells was calculated. Representative crystal violet stained images are shown below for each cell line after 7 days (due to a dramatic decrease in cell viability we were unable to harvest enough live cells to confirm TSPAN1 knockdown after 96 hours). (C) Western blot analysis of various proteins in LNCaP and (D) CWR22 RV1 cells following siRNA mediated protein depletion of TSPAN1 for 96 hours. Please note: cropped western blots are displayed in the figure, but full length blots are included in the Supplementary Information file.