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. 2017 Jul 12;7:5166. doi: 10.1038/s41598-017-04091-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PFOS treatment results in dysbiosis at late phase of C. rodentium infection. Wild-type mice were treated with PFOS and infected with C. rodentium as described in Fig. 1. (A,C and E) The expression of indicated commensals in the feces of PFOS treated mice and control at day 5 post C. rodentium infection (p.i.) was analyzed by real-time PCR. (B,D and F) The expression of indicated commensals in the feces of PFOS treated mice and control at day 11 post C. rodentium infection (p.i.) was analyzed by real-time PCR. Statistical analyses were performed using Mann-Whitney unpaired U test. Error bars represent SEM. Data were pooled from three independent experiments.