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. 2017 Jul 12;7:5259. doi: 10.1038/s41598-017-05693-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative SEM images showing the effect of laser irradiation on E. coli cells attached to: (by column) (a,d,g) Control glass; (b,e,h) Type II surfaces (glass|GNS|SiO₂); (c,f,i) Type III surfaces (glass|GNS|SiO₂|AgNP). Increasing power density (by row): (a,b,c) = non irradiated samples; (d,e,f) = samples irradiated with 5× objective lens; (g,h,i) = 20× objective lens. All scale bars are 500 nm. Large area SEM images are shown in ESI (SI14 and SI15a).