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. 2017 Jul 12;7:5256. doi: 10.1038/s41598-017-05426-6

Figure 4.

Figure 4

EDL FET Biosensors for CRP detection. Purified proteins prepared in 1X PBS with 1% BSA and clinical human sera are tested using HEMT integrated with microfluidic device. (a) Aptamer based detection in time domain for CRP prepared in 1X PBS with1% BSA. Concentration dependent drain current increase is observed. (b) Accumulated charges vs. time for each CRP concetration. Signal to noise ratio is improved by using total charge as the index as random noise is cancelled out. (c) Calibration curve for CRP detection in 1X PBS with 1% BSA. The measured CRP concentrations are 1 fM, 10 fM, 100 fM, 10 pM, 2.6 nM, 9 nM, 26 nM and 100 pM. (d) CRP detection in time domain for clinical human serum samples. (e) Accumulated charges for different CRP concentrations in serum. (f) Calibration curve for CRP detection in serum. The measured CRP concentrations are 0.34 mg/L, 0.36 mg/L, 0.38 mg/L, 0.65 mg/L, 1.37 mg/L, 1.5 mg/L, 2.3 mg/L, 5.4 mg/L 9.2 mg/L 23.2 mg/L.