Figure 2.

F991 attenuated the inflammatory progression of DSS-induced colitis (A) Colon lysates from WT mice were extracted by TSD lysis buffer, and then purified by the F991-coupled column, using the control peptide-coupled column as the control column. Input, elution were analyzed by western blot and SDS-PAGE under non-reducing condition and reducing condition with anti-Igκ and anti-Igλ antibody. One representative experiment of two is displayed. (B) and (C) The body weight and disease activity index of the mice were measured when the mice were treated with 4% DSS in their drinking water for 8 days to induce acute colitis, and F991 (15 mg/kg) was administered daily via i.p. injection. In addition, the same volume of PBS was administered through i.p. in F991-vehicle mice. (D) The length of the colon was measured when the mice were sacrificed on the 2^nd, 4^th or 6^th day. (E) Histology score of the severity of inflammatory lesions was evaluated after treatment of F991 when the mice were sacrificed on 6^th day. (F) The length of the colon and the (H & E) staining are shown after F991 treatment. Scale bars: 50 μM. (G–I) All leukocytes (CD45⁺ cells), mast cells, macrophages, neutrophils and subpopulations of DCs in the LP, were isolated and counted on the 2^nd, 4^th and 6^th day by flow cytometric assay. The results (B–I) are shown as the mean ± SD of 4 mice per group at each time point. ns: no statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001 compared with group DSS + PBS. Con: control peptide, DSS + PBS: mice challenged with DSS and injected with PBS, DSS + F991: mice challenged with DSS and injected with F991. One representative experiment of three is displayed.