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. 2017 Jul 12;7:5254. doi: 10.1038/s41598-017-05762-7

Figure 1.

Figure 1

Osthole reverses defective osteogenic ability of P-PDLSCs. (A) ALP staining and ARS staining after 7 days (ALP staining) and 21 days (ARS staining) in H-PDLSCs and P-PDLSCs with different concentrations of Osthole (0 Mol/L, 10−4 Mol/L, 10−5 Mol/L, 10−6 Mol/L, 10−7 Mol/L and 10−8 Mol/L). (B) Quantification of ARS staining for light absorbance at 562 nm. (C) Gene expression of Runx2, ALP and Osterix in H-PDLSCs, P-PDLSCs and P-PDLSCs with 10−7 Mol/L Osthole as assayed by qRT-PCR. (D) Protein expression of Runx2, ALP and Osterix in H-PDLSCs, P-PDLSCs and P-PDLSCs with 10−7 Mol/L Osthole as assayed by Western blot. *P < 0.05, **P < 0.01, ns: P ≧ 0.05, n = 3.