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. 2017 Jul 12;7:5254. doi: 10.1038/s41598-017-05762-7

Figure 3.

Figure 3

Osteogenic differentiation of H-PDLSCs with MOZ & MORF depletion. (A) Protein expression of MOZ, H3K9ac and H3K14ac in H-PDLSCs and H-PDLSCs with siMOZ. (B) Gene expression of Runx2, ALP and Osterix in H-PDLSCs and H-PDLSCs with siMOZ after osteogenic induction. (C) Protein expression of Runx2, ALP and Osterix in H-PDLSCs and H-PDLSCs with siMOZ after osteogenic induction. (D) ARS staining of H-PDLSCs and H-PDLSCs with siMOZ and quantification of it for light absorbance at 562 nm. (E) Protein expression of MORF, H3K9ac and H3K14ac in H-PDLSCs and H-PDLSCs with siMORF measured by western blot. (F) Gene expression of Runx2, ALP and Osterix in H-PDLSCs and H-PDLSCs with siMORF after osteogenic induction. (G) Protein expression of Runx2, ALP and Osterix in H-PDLSCs and H-PDLSCs with siMORF after osteogenic induction. (H) ARS staining of H-PDLSCs and H-PDLSCs with siMORF and quantification of it for light absorbance at 562 nm. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3.