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. 2017 Jul 12;7:5254. doi: 10.1038/s41598-017-05762-7

Figure 4.

Figure 4

Osthole improves the formation of P-PDLSC sheet. (A) Representative macroscopic and microscopic images of H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole. (B) Representative H & E staining of H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole, and mean thickness of statistical analysis was shown below. Scale Bar, 50 μm (upper) and 20 μm (lower). (C) Representative SEM images of H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole. Scale Bar, 300 μm (upper) and 60 μm (lower). (D) Gene expression of Fibronectin, Integrin and Periostin in H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole as assayed by qRT-PCR. (E) Protein expression of Fibronectin, Integrin and Periostin in H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole as assayed by western blot. *P < 0.05, **P < 0.01, ***P < 0.001, ns: P ≧ 0.05, n = 3.