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. 2017 Jul 12;7:5254. doi: 10.1038/s41598-017-05762-7

Figure 5.

Figure 5

Osthole improves osteogenesis of P-PDLSC sheet. (A) ALP staining and ARS staining after 7 days (ALP staining) and 21 days (ARS staining) in H-PDLSC and P-PDLSC sheets with different concentrations of Osthole. (B) Quantification of ARS staining for light absorbance at 562 nm. (C) Gene expression of Runx2, ALP and Osterix in H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole. (D) Protein expression of Runx2, ALP and Osterix in H-PDLSC sheet, P-PDLSC sheet and P-PDLSC sheet with 10−7 Mol/L Osthole. */#P < 0.05, **/##P < 0.01, ***P < 0.001, ns: P ≧ 0.05, n = 3.