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. 2017 Jul 12;7:5204. doi: 10.1038/s41598-017-05580-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative real-time PCR validation of microarray expression data of ATP6V1A and LINC00837 for the TEXUS-51 suborbital rocket flight experiment. RNA samples used fo the microarray analysis were analyzed additionally by quantitative real time PCR as second independent method. (a) Microarray analysis: Significantly differential gene expression of ATP6V1A could be confirmed in microgravity (µg) versus 1 g in - flight (1 gIF) and in hypergravity (baseline BL) versus 1 g in - flight (1 gIF). Significantly differential gene expression of LINC00837 could be confirmed in microgravity (µg) versus 1g in - flight (1gIF). (b) Quantitative real time PCR analysis. Significant differential gene expression value of the same magnitude were detected in quantitative real-time PCR analysis. Statistical significance was tested by ANOVA for microarray results and by T-test in case of qRT-PCR, n = 9 for 1g in - flight (1gIF), n = 7 for baseline (BL) and n = 9 for ug.